What is the readout of an E-test on agar?

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Prepare for your Antimicrobial Susceptibility Testing and Rapid Diagnostics exam. Engage with flashcards and multiple choice questions, each supplemented by hints and thorough explanations. Boost your confidence and readiness for the exam!

Multiple Choice

What is the readout of an E-test on agar?

Explanation:
An E-test readout is the inhibition boundary around the gradient antibiotic strip. The strip releases a continuous range of concentrations into the agar, so bacteria are inhibited where the local concentration meets the organism’s MIC. This creates a curved, typically drop-shaped ellipse of inhibition that wraps around the strip. Reading the MIC is done at the point where this edge intersects the scale on the strip, which gives the MIC value in the strip’s units. This differs from disk diffusion (zone diameter), broth turbidity (broth tests), or color-change readouts.

An E-test readout is the inhibition boundary around the gradient antibiotic strip. The strip releases a continuous range of concentrations into the agar, so bacteria are inhibited where the local concentration meets the organism’s MIC. This creates a curved, typically drop-shaped ellipse of inhibition that wraps around the strip. Reading the MIC is done at the point where this edge intersects the scale on the strip, which gives the MIC value in the strip’s units. This differs from disk diffusion (zone diameter), broth turbidity (broth tests), or color-change readouts.

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